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成果归属企业: 深圳大学

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成果介绍

本发明公开了一种荧光差分超分辨成像方法及成像系统。通过调整系统中角反射器的位置延长或缩短一束高斯脉冲激光在第一光路中传播的光程并转换为环形脉冲激光后聚焦照射样品，另一束高斯脉冲激光沿第二光路传播聚焦照射样品，照射样品的环形脉冲激光与高斯脉冲激光之间的脉冲间隔大于荧光染料的荧光寿命，通过两个探测器分别采集得到激发光脉冲信号及荧光信号。从荧光信号中分离出第一图像及第二图像，根据图像处理规则对第一图像及第二图像进行分析处理后得到分辨率进一步提升的目标超分辨图像。通过上述方法，采用低功率的脉冲激光减小了对生物样品的破坏，解决了传统荧光差分超分辨成像时因像素失配导致超分辨图像质量下降的问题。

成果应用案例介绍

光学工程>成像>超分辨成像方法

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