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基于静态帧的部件运动性预测方法

成果领域:

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成果归属企业: 深圳大学

成果鉴定时间:

成果介绍

本发明公开一种四环素慢病毒诱导表达载体及其建立方法和应用，所述四环素慢病毒诱导表达载体包含目的基因表达框和反义四环素转录激活因子表达框；诱导型CRISPR/Cas9系统将Cas9蛋白置于诱导型启动子的下游，并将U6启动表达的sgRNA序列置于整个四环素诱导体系的上游。本发明首次提出TRE3Gp与TetON3G的组合效果最佳，该诱导体系引入细胞后可在保持低背景表达的同时，最大水平的诱导表达目的基因。该诱导系统对诱导剂反应灵敏、效率高、分子量小，可广泛应用于基因功能研究中。

成果应用案例介绍

医药医疗>基因相关>基因功能; 方法过程>构建方法>构建; 机械设备>其它机械设备类>crispr/cas9系统; 生物(体)>动物组织>诱导表达载体; 生物(体)>生物病毒>四环素慢病毒

联系方式

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邮箱：zeccea@qq.com QQ群：318441538 客服电话：0758-2721389 秘书处电话/微信：13822630888

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[ BEHAVIOR ] Run Closure @app_init [ RunTime:0.000012s ]
[ CACHE ] INIT File
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[ BEHAVIOR ] Run \addons\treaty\Treaty @app_init [ RunTime:0.000029s ]
[ BEHAVIOR ] Run \addons\upyun\Upyun @app_init [ RunTime:0.000015s ]
[ BEHAVIOR ] Run Closure @app_init [ RunTime:0.000031s ]
[ BEHAVIOR ] Run app\common\behavior\Common @app_init [ RunTime:0.000245s ]
[ LANG ] /www/wwwroot/open.juabc.com/thinkphp/lang/zh-cn.php
[ BEHAVIOR ] Run app\common\behavior\Common @app_dispatch [ RunTime:0.000013s ]
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[ BEHAVIOR ] Run app\common\behavior\Common @module_init [ RunTime:0.000687s ]
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[ DB ] INIT mysql
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[ RUN ] app\index\controller\Results->views[ /www/wwwroot/open.juabc.com/application/index/controller/Results.php ]
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[ BEHAVIOR ] Run \addons\wxfollowlogin\Wxfollowlogin @view_filter [ RunTime:0.000044s ]

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