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一种混凝土防护剂、混凝土防护膜及其制备方法

成果领域:

推广方式:

成果归属企业: 深圳大学

成果鉴定时间:

成果介绍

本发明公开了一种基于蓝光诱导的衣藻外源基因表达系统及其应用，所述表达系统的元件包括光受体隐花色素CRY2基因及其相互作用蛋白CIB1基因、GAL4的DNA结合结构域BD和具有转录激活活性单纯疱疹病毒的激活域VP64、核定位序列NLS、能与GAL4转录因子DNA结合结构域BD结合的UAS序列、外源基因，其中CRY2‑BD、CIB1‑VP64‑NLS、UAS‑外源基因分别连接在一起，并通过遗传转化整合到衣藻的核基因组中。本发明以单色光‑蓝光作为诱导外源基因表达的诱导因素，克服了热激诱导对藻细胞的伤害, 也克服了各种化学诱导剂对藻细胞代谢的影响。

成果应用案例介绍

机械设备>其它机械设备类>基因表达系统

联系方式

电话/微信: 13822630888

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邮箱：zeccea@qq.com QQ群：318441538 客服电话：0758-2721389 秘书处电话/微信：13822630888

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[ BEHAVIOR ] Run Closure @app_init [ RunTime:0.000014s ]
[ CACHE ] INIT File
[ BEHAVIOR ] Run \addons\briefurl\Briefurl @app_init [ RunTime:0.001366s ]
[ BEHAVIOR ] Run \addons\fastflow\Fastflow @app_init [ RunTime:0.000026s ]
[ BEHAVIOR ] Run \addons\notice\Notice @app_init [ RunTime:0.000250s ]
[ BEHAVIOR ] Run \addons\qrcode\Qrcode @app_init [ RunTime:0.000192s ]
[ BEHAVIOR ] Run \addons\treaty\Treaty @app_init [ RunTime:0.000030s ]
[ BEHAVIOR ] Run \addons\upyun\Upyun @app_init [ RunTime:0.000015s ]
[ BEHAVIOR ] Run Closure @app_init [ RunTime:0.000032s ]
[ BEHAVIOR ] Run app\common\behavior\Common @app_init [ RunTime:0.000249s ]
[ LANG ] /www/wwwroot/open.juabc.com/thinkphp/lang/zh-cn.php
[ BEHAVIOR ] Run app\common\behavior\Common @app_dispatch [ RunTime:0.000013s ]
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[ HEADER ] array ( 'connection' => 'keep-alive', 'cache-control' => 'max-age=0', 'x-forwarded-for' => '10.3.33.245', 'via' => '1.1 squid-proxy-5b96dc6d46-rd877 (squid/6.13)', 'host' => 'www.zqqyzhfw.com', 'accept-encoding' => 'gzip, br, zstd, deflate', 'user-agent' => 'Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)', 'accept' => '*/*', 'content-type' => '', 'content-length' => '', )
[ PARAM ] array ( 'ids' => '4935', )
[ LANG ] /www/wwwroot/open.juabc.com/public/../application/index/lang/zh-cn.php
[ BEHAVIOR ] Run app\common\behavior\Common @module_init [ RunTime:0.000677s ]
[ BEHAVIOR ] Run \addons\upyun\Upyun @module_init [ RunTime:0.000115s ]
[ BEHAVIOR ] Run \addons\wxfollowlogin\Wxfollowlogin @module_init [ RunTime:0.000018s ]
[ BEHAVIOR ] Run \addons\upyun\Upyun @upload_config_init [ RunTime:0.000354s ]
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[ BEHAVIOR ] Run \addons\notice\Notice @config_init [ RunTime:0.000022s ]
[ DB ] INIT mysql
[ BEHAVIOR ] Run \addons\csmtable\Csmtable @action_begin [ RunTime:0.000039s ]
[ RUN ] app\index\controller\Results->views[ /www/wwwroot/open.juabc.com/application/index/controller/Results.php ]
[ VIEW ] /www/wwwroot/open.juabc.com/public/../application/index/view/results/views.html [ array ( 0 => 'user', 1 => 'site', 2 => 'config', 3 => 'data', 4 => 'active', 5 => 'login', 6 => 'catelist', 7 => 'views', ) ]
[ BEHAVIOR ] Run \addons\wxfollowlogin\Wxfollowlogin @view_filter [ RunTime:0.000039s ]

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